FERMENTATIVE ACTIVITY OF FIVE STRAINS OF NEOCALLIMASTIX FRONTALIS CULTIVATED ON A DIFFERENT SUBSTRATES
Natalija Atanasova-Pančevska, Djoko Kungulovski
Department of Microbiology and Microbial Biotechnology, Institute of Biology, Faculty of Natural Sciences and Mathematics, Arhimedova 5, Skopje, Republic of Macedonia
natalijaap@gmail.com
The importance of the microbial ecology and diversity of microorganisms in the digestive tract of herbivora has gained increasing attention in response to recent trends in the global livestock production. The microorganisms in the digestive tracts of ruminants and non-ruminant herbivora have a profound influence on the conversion of feed into end-products, which can impact on the animals and environment. Anaerobic fungi are the significant constituent of rumen microbiota in livestock that rely on poor-quality fibrous diets. They produce the whole set of enzymes necessary for plant cell-wall degradation. These enzymes enable fungi to penetrate plant cell walls, access fermentable substrates not available to surface-acting bacteria, colonize the sturdy plant structures, weaken and degrade plant tissues and reduce the plant particles size. The present paper studies the influence of different carbohydrate substrates on fermentative activity of five strains of Neocallimastix frontalis. Experiments for fermentative activity were performed with carboxymethyl cellulose, Whatman No1 and Avicel, and enzyme activity was detected extracellularly in culture supernatants, through reducing sugars, after vegetative growth. The enzymes CM-cellulаse, FP-ase and avicelase secreted from N. frontalis strain J3 released the largest quantity of reducing sugars, compared to the other strains.
Keywords: anaerobic fungi; Neocallimastix frontalis; herbivora; ruminant; enzymesovi
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