EXAMINATION OF AFLATOXINS B1 AND G1 IN FEED
Julijana Tomovska, Velina Stefanovska, Vesna K. Hristova, Nikola Georgievski
University "St. Climent Ohridski", Faculty of Biotechnical Sciences, Bitola, R. Makedonia
dzulitomovska@yahoo.com
Aflatoxins B1 (AFB1) and G1 (AFG1) are micotoxins derived from molds Aspergillus flavus and Aspergillus parasiticus present in animal food stored in an environment with relative humidity of 85%, moisture content of the surface about 30%, temperature of 25oC and a suitable substrate. Tests for AFB1 and AFG1 are made in 60 feed samples (corn, concentrate and silage) from 20 locations. Two methods have been used, Charm II 6600 Luminometer where aflatoxins fluorescent strongly in ultraviolet light of 365 nm, B1 generates a blue fluorescent and G1 green fluorescent, and with VICAM where AFB1 are extracted in portions with acetonitrile/methanol, and application of HPLC. In corn C(AFB1)/ppb with Charm II 6600 Luminometer was 3.5±2.0647; in concentrate 3.25±1,8883, and in silage 6.45 ±2.0124; for C(AFG1)/ppb was: 3.2±1.9358, 2.9±1.9324, and 5.7±2.22663, respectively. In the same samples C(AFB1)/ppb determinate with VICAM are: 4.49±2.3548, 4.93±2.7941 and 7.73±1.766054. The results obtained by HPLC are with two decimal places and for a unit higher. Aflatoxins present in the feed used by the Pelagonian region vary within the limits of normal (0‑10 ppb) except silage which has a higher value due to its greater exposure to mold. Determining the concentration of AFB1 and AFG1 in animal feed is of great importance, the feed with aflatoxins results in high toxicity, carcinogenicity and mutagenicity and affects the health of livestock and their application in nutrition and health of people.
Keywords: aflatoxins; aflatoxin B1; aflatoxin G1; animal feed
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