BACILLUS
CEREUS AND SELENIUM HAVE GOOD EFFECT ON IN VITRO MATURATION OF OOCYTES
Tatjana Smiljaković, Ljiljana Stojanović, Snežana
Trenkovski, Marija Kojić,
Čedomir Radović, Ljiljana Sretenović, Stevica Aleksić
Institute for Animal Husbandry,
Autoput 16, P. Box 23, 11080, Belgrade–Zemun,
Republic of Serbia
smiljakovic@gmail.com
It is well known that selenium has positive effect on
the reproductive tract either in female or male individuals, but cytology
and biochemistry are not well elucidated. In this paper cytologically and
microbiologically is shown that bacteria Bacillus cereus and selenium have large positive and
synergistic influence on maturation of oocytes in vitro. This is caused by the bactericide effect on
coliform bacteria which are abundant in the digestive tract. Taking
account that anus and vagina are often nearby each other and that even
sterility can be caused by coliform bacteria in the upper parts of the
female reproductive tract, we found microbiologically and cytologically
in oocyte in the presence of coliform bacteria, and Bacillus cereus and selenium, that number of coliform
bacteria decreases, and cytologically oocytes cultured in such medium in vitro have more vitality than
in culture medium without at least selenium supplement. These
investigations should be continued with the aim of optimization of
conditions for maturation of oocytes and fertilization in vitro for embryo transfer of
domestic animals as well as model for in
vivo reproduction.
Key words: reproduction,
oocyte; in vitro maturation (IVM); Bacillus cereus; selenium; coliform
bacteria
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