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THE DGAT-1
GENE POLYMORPHISM IS INFORMATIVE QTL MARKER FOR MEAT QUALITY IN BEEF
CATTLE
Piotr
Urtnowski, Jolanta Oprzadek, Adrianna Pawlik, Edward Dymnicki
Polish Academy of Sciences Institute of Genetics and Animal Breeding,
Jastrzebiec, 05-552 Wolka Kosowska, Poland
email: p.urtnowski@ighz.pl
Acyl-CoA:diacylglycerol acyltransferase-1 (DGAT-1) is
a key enzyme involved in triglyceride synthesis. DGAT-1 gene is located
in the centromeric region of the bovine chromosome (BTA) 14 and
considered polymorphism was identified as a candidate gene for milk and
meat QTL. One of mutations which is substitution AA → GC in exon 8 causes
an amino acid change in the product. The effect of the lysine/alanine
(K232A) diallelic polymorphism on meat production traits has been
studied. 156 young Black-and-White (Friesian) bulls were genotyped. The
association between diacylglycerol acyltransferase polymorphism and
slaughter performance and meat technology analysis were examined. Moreover
the fatty acid profile (C12–C20:5) including CLA in the sample of
longissimus dorsi (LD) muscle was evaluated. Differences (P ≤ 0,05) were
found between genotypes in slaughtering performance traits as well as fat
and valuable cuts content and meat-fat ratio. The significant difference
occurred also in the water holding capacity. Heterozygous individuals
AA:GC were characterized by the greatest values compared with homozygous
ones. Bulls of DGAT-1 genotype GC:GC showed significantly higher (P ≤
0,05) content of lauric acid in LD muscle and heterozygous animals differ
in CLA content with homozygous AA:AA.
Key words: DGAT-1,
cattle, beef, gene polymorphism, carcass traits
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