EFFECT OF GLYCEROL SUPPLEMENTED DIET AT DIFFERENT AGES ON JAPANESE QUAILS ON CECAL MICROFLORA
Onder A. Ustundag, Ahmet E. Tuzun, Mursel Ozdogan, Gamze Basbulbul Ozdemir
dnan Menderes University Faculty of Agriculture, Department of Feed and Animal Nutrition, South Campus, Aydïn, Turkey
austundag@adu.edu.tr
This study evaluated the effects on the bacterial microflora in the cecum of Japanese quail caused by the addition of crude glycerol to their diet at different age. In this experiment, 480-day-old Japanese quails were used. Quails were assigned randomly into four dietary groups. The diets containing no glycerol (control diet) and 5% glycerol were used in the experiment. The first group (Group I) was fed with the control diet. Group II between 1-35-day-old, quails group III between 1-21-day-old, quails and group IV between 21-35-day-old quails were fed with the diet containing 5% glycerol. At the same time, Group III and Group IV consumed control diet at the age of 22-35-days and 1-20-days, respectively. The experiment lasted 35 days. Cecum samples were collected from a total of 128 quails (16 female and 16 male in each group) at the age of 21- and 35-days. The amount of enterobacteria, coliform, and total bacteria was determined in the cecum. The groups of enterobacteria and coliform bacteria in the cecum of 21-day-old females and 35-day-old male quails only showed statistical difference among the groups. On the other hand, it was seen that the numbers of total bacteria of male (P<0.01) and female (P<0.05) quails at the age of 1 to 35 days fed with diet containing 5% glycerol between 21 and 35 days were significant in comparison to the control group of 35 days-old quails.
Keywords: glycerol; quail; bacterial microflora; cecum
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