VIRULENCE GENES AND BIOTYPE PREVALENCE ASSOCIATED WITH AVIAN ESCHERICHIA COLI PATHOGENICITY, IN ALBANIAN POULTRY FARMS
Tana Shtylla1, Ilirian Kumbe1, Pranvera Çabeli2, Antonio Camarda2
1Faculty of Veterinary Medicine, Agriculture University of Tirana, Albania
2Facoltà di Medicina Veterinaria, Università di Studi Di Bari, Italy
shtylla2003@yahoo.com
101 avian pathogenic Escherichia coli (APEC) strains from colibacillosis affected poultry were tested for the presence of eight different virulence associated genes. Besides, the APEC strains were biotyped. The association of belonging biotype to the corresponding pathotype was studied basing on the breeding type. The most frequent genes detected by means of PCR-multiplex were iss, Irp2 and IucD (43.56%, 41.58% and 48.51%, respectively). Meanwhile their presence was significantly more relevant in the intensive poultry farms strains. The most pathogenic pathotype was considered the one with the combination of seven genes: astA-iss-irp2-iucD-papC-vat-cva A/B, with a presence of 5.45% in the intensive farms originating strains and at 0% in the rural type strains. The absence of virulence genes in the pathotype was seen mostly in the rural type strains 43.47% comparing it with the 21.82% in the intensive farms. The most frequent biotypes strains were B 28, B30 and B 31 with 13.86%, 16.83% and 15.84% each. Nevertheless the corresponding presence of these biotypes was more relevant with pathogenic strains originating from intensive breeding poultry farms. No data on the prevalence and the association of poultry E.coli biotypes with pathotypes was studied previously for evaluating the real potential pathogenic attitude of the bacterium.
Keywords: APEC; biotype; pathotype; PCR-multiplex; poultry
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