PROTEOMIC STUDY: LIVER METABOLISM THROUGH A COMPARISON OF THE PROTEIN EXPRESSION PROFILES OF THE TWO BREEDS – CHIANINA AND HOLSTEIN
Leonardo Murgiano1, Anna Maria Timperio2, Lello Zolla2, Silvia Bongiorni1, Alessio Valentini1, Lorraine Pariset1
1Dipartimento di Produzioni Animali, Universita della Tuscia, Viterbo, Italy
2Dipartimento di Scienze Ambientali, Universita della Tuscia, Viterbo, Italy
lmurgiano@unitus.it
The Bos taurus breeds Holstein and Chianina are representatives of the extreme selection mainly for milk and meat traits. In the present study, we wanted to assess if selection for different purposes had effect into liver metabo¬lism by comparing protein expression profiles of the two breeds. We applied two-dimensional polyacrylamide gel electrophoresis (2DE) to identify proteins which are differentially expressed in the two breeds. A total of 560±57 spots were matched between the Chianina and the Holstein. Thirty nine differential expressed proteins were charac¬terized between the two breeds. Taken together, our study performed at the proteomic level clearly supports deep changes in metabolism induced by human selection in the Holstein and the Chianina. Differential proteomic profiles of two bovine livers were established and analyzed by means of immobilized pH gradient-based two-dimensional polyacrylamide gel electrophoresis (2D IEF-SDS- PAGE) and electrospray/ionization time of flight mass spectrome¬try (qTOF-MS). For the analysis a sampling of 12 animals (six Chianina and six Holstein) was considered. For each animal the 2D electrophoresis protein extraction step was performed separately. Three technical replicates were run to reduce the technical variance for each average sample. Interestingly, most of the proteins found up-regulated as well as newly expressed (on) in the Chianina with respect to Holstein resulted involved in the anabolic pathways. On the contrary, newly expressed proteins in Holstein were related with hormone homeostasis and in thermoregulatory ability. In Holstein we found an overexpressed protein belonging to sulfotransferase (SULT1C2) family that plays a key role in biosynthesis and homeostasis of a number of hormones, including estrogens and iodothyronines.
Keywords: liver proteins; proteomics; cattle breeds; 2D-IEF-SDS-PAGE; MS/MS
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